Studies on the cyanobacterial family C DNA polymerase
نویسندگان
چکیده
منابع مشابه
Further studies on partially purified calf thymus DNA polymerase a.
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15 صفحه اولPlasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the excised strand remains annealed to the plasmid through non-covalent Watson-Crick base-pa...
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The preparation of a gapped pUC18 derivative, containing the lacZa reporter gene in the singlestranded region, is described. Gapping is achieved by flanking the lacZa gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the excised strand remains annealed to the plasmid through non-covalent Watson–Crick base-pairing; it...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1998.tb12797.x